GSM4053609: A549 cells, Input rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
A549 cells
cell line
A549
cell type
lung epithelial adenocarcinoma cell line
passages
18-25
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and ELF1-DNA complexes were isolated with antibody. Input or ChIPped DNA was end-repaired and "A" bases were subsequently added to the DNA. After purification, Illumina/Solexa PE adapters were ligated to DNA fragments. The DNA fragments were then purified and subjected to PCR using Illumina/Solexa primers PE 1.0 and 2.0. These final ChIP-Seq libraries were then purified with the QIAGEN MinElute kit.