Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF7 cells
genotype
wild type
chip antibody
anti-ERα, Cell Signaling Technology, 8644S

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Library preparation and sequencing procedures were carried out as described previously according to Illumina protocols with minor modifications (Illumina, San Diego).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
9291211
Reads aligned (%)
83.5
Duplicates removed (%)
6.7
Number of peaks
3589 (qval < 1E-05)

hg38

Number of total reads
9291211
Reads aligned (%)
84.1
Duplicates removed (%)
6.4
Number of peaks
3522 (qval < 1E-05)

Base call quality data from DBCLS SRA