Antigen

Antigen Class

TFs and others

Antigen

ESR1

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MCF7 cells

genotype

wild type

chip antibody

anti-ERα, Cell Signaling Technology, 8644S

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Library preparation and sequencing procedures were carried out as described previously according to Illumina protocols with minor modifications (Illumina, San Diego).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

9291211

Reads aligned (%)

89.9

Duplicates removed (%)

6.4

Number of peaks

3522 (qval < 1E-05)

hg19

Number of total reads

9291211

Reads aligned (%)

89.5

Duplicates removed (%)

6.7

Number of peaks

3589 (qval < 1E-05)