GSM4044324: ERa ChIP-seq E2 treatment DTFF1 persistent site Rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ESR1
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MCF7 cells with PS deletion
treatments
Estradiol 1 hr
antibody
sc-8002 and ab32063
cell type
MCF7 cells with PS deletion
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq, ERa bound regions were pull down using antibody. 5C, chromatin interactions were detected by 5C Libraries were prepared using standard ChIP-seq library protocol For 5C, Set of forward and reverse oligos with T7 and T3 adaptors were anneled to 3C libraies. The ligated oligos were amplified and sequenced on illumina Hiseq 2500 platform