Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Lam

Cell type

Cell type Class
Cell line
Cell type
S2
Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by original data submitter

Sample

source_name
embryonic cells
cell line
S2
chip antibody
lamin L7 antibody
genotype/variation
wild type

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates from embryonic cells or 3rd instar brains were clarified from sonicated nuclei and lamin-DNA complexes were isolated with specific antibody Libraries were prepared according to Illumina's instructions for HiSeq 2000 for KC or S2 cells and NextSeq 500/550 High Output Kit when brains were analyzed.

Sequencing Platform

instrument_model
NextSeq 500

dm3

Number of total reads
15479041
Reads aligned (%)
46.5
Duplicates removed (%)
9.9
Number of peaks
1871 (qval < 1E-05)

dm6

Number of total reads
15479041
Reads aligned (%)
45.4
Duplicates removed (%)
11.5
Number of peaks
2032 (qval < 1E-05)

Base call quality data from DBCLS SRA