Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Lam

Cell type

Cell type Class
Cell line
Cell type
Kc167
Source
e/se
Developmental Stage
dorsal closure stage

Attributes by original data submitter

Sample

source_name
embryonic cells
cell line
KC
chip antibody
lamin L7 antibody
genotype/variation
wild type

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates from embryonic cells or 3rd instar brains were clarified from sonicated nuclei and lamin-DNA complexes were isolated with specific antibody Libraries were prepared according to Illumina's instructions for HiSeq 2000 for KC or S2 cells and NextSeq 500/550 High Output Kit when brains were analyzed.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
16212016
Reads aligned (%)
36.9
Duplicates removed (%)
7.6
Number of peaks
615 (qval < 1E-05)

dm6

Number of total reads
16212016
Reads aligned (%)
36.8
Duplicates removed (%)
9.2
Number of peaks
1730 (qval < 1E-05)

Base call quality data from DBCLS SRA