Antigen

Antigen Class

TFs and others

Antigen

ZNF398

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hES BG01V

Primary Tissue

Embryo

Tissue Diagnosis

Normal

Sample

source_name

BG01V hESCs

cell type

BG01V

antibody

ZNF398

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

After decrosslinking, DNA was purified using QIAQuick PCR Purification Kit (Qiagen) according to the manufacturer's instructions. ChIP-seq library was prepared with approximately 5 ng of immunoprecipitated DNA were used as input for the NEBNext® ChIP-Seq Library Prep kit, following manufacturer's instructions.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

27019025

Reads aligned (%)

89.5

Duplicates removed (%)

49.5

Number of peaks

1079 (qval < 1E-05)

hg19

Number of total reads

27019025

Reads aligned (%)

88.7

Duplicates removed (%)

51.5

Number of peaks

868 (qval < 1E-05)