Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ZNF398

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hES BG01V
Primary Tissue
Embryo
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
BG01V hESCs
cell type
BG01V
antibody
ZNF398

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
After decrosslinking, DNA was purified using QIAQuick PCR Purification Kit (Qiagen) according to the manufacturer's instructions. ChIP-seq library was prepared with approximately 5 ng of immunoprecipitated DNA were used as input for the NEBNext® ChIP-Seq Library Prep kit, following manufacturer's instructions.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
27019025
Reads aligned (%)
43.0
Duplicates removed (%)
51.5
Number of peaks
868 (qval < 1E-05)

hg38

Number of total reads
27019025
Reads aligned (%)
45.2
Duplicates removed (%)
49.5
Number of peaks
1079 (qval < 1E-05)

Base call quality data from DBCLS SRA