Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TP53

Cell type

Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
HCT 116
cell type
colorectal carcinoma cells
chip antibody
Anti-p53 AB7 (Calbiochem)
cell line
HCT 116

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
10 ng of the immunoprecipitated chromatin was used to prepare the libraries for sequencing following the manufacturer’s instructions including DNA end repairing, adaptor ligation, and amplification

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
17621454
Reads aligned (%)
53.2
Duplicates removed (%)
46.1
Number of peaks
543 (qval < 1E-05)

hg38

Number of total reads
17621454
Reads aligned (%)
54.0
Duplicates removed (%)
45.4
Number of peaks
536 (qval < 1E-05)

Base call quality data from DBCLS SRA