Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Pancreas

Cell type

Pancreas

MeSH Description

A nodular organ in the ABDOMEN that contains a mixture of ENDOCRINE GLANDS and EXOCRINE GLANDS. The small endocrine portion consists of the ISLETS OF LANGERHANS secreting a number of hormones into the blood stream. The large exocrine portion (EXOCRINE PANCREAS) is a compound acinar gland that secretes several digestive enzymes into the pancreatic ductal system that empties into the DUODENUM.

Sample

source_name

pancreatic tissue

strain

C57BL/6

gender

male

treatment

4 hours after the initiation of CIP

antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin samples were prepared using pancreatic tissue obtained from 2-4 months old mice injected with cerulein. Chromatin Immunoprecipitation was carried out using Anti-ATF3 (sc-188; Santa Cruz). After reverse cross-linking and treatment with Proteinase K, the samples were treated with Phenol Chloroform twice. The DNA was Ethanol precipitated, washed with 70% Ethanol. Samples were eluted and pooled in 40ul of H2O. This eluted DNA was sent for sequencing. Library was constructed by the Beijing Genomics Institute (Hong Kong), following their established protocol (http://www.genomics.cn/en/).

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

mm10

Number of total reads

12579897

Reads aligned (%)

98.3

Duplicates removed (%)

7.1

Number of peaks

184 (qval < 1E-05)

mm9

Number of total reads

12579897

Reads aligned (%)

98.2

Duplicates removed (%)

7.2

Number of peaks

188 (qval < 1E-05)