Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TRIM28

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
embryonic stem cells, KAP1 ChIP
transchromosomic
no
plasmid
none
chip_or_input
KAP1 ChIP
chip antibody
KAP1 ChIP (abcam ab10483, lot#GR17300)
nickname
KAP1 (hESC) r3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
O'Geen H, Echipare L, Farnham PJ. Using ChIP-seq technology to generatehigh-resolution profiles of histone modifications. Methods Mol Biol. 2011;791:265-86. doi: 10.1007/978-1-61779-316-5_20. PubMed PMID: 21913086. TrueSeq DNA library prep, low input protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
16171785
Reads aligned (%)
51.4
Duplicates removed (%)
71.1
Number of peaks
213 (qval < 1E-05)

hg38

Number of total reads
16171785
Reads aligned (%)
52.7
Duplicates removed (%)
70.5
Number of peaks
242 (qval < 1E-05)

Base call quality data from DBCLS SRA