GSM1466834: Human ESCs IgG MS417- treated 6h 02; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC H9
NA
NA
Attributes by original data submitter
Sample
source_name
human H9 cell lines
cell line
H9
chip antibody
IgG Santa Cruz (2027)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNeasy kit (Qiagen) for RNA-seq; 1% formaldheyde crosslinked chromatin for ChIP-seq Poly-A+ selection (magnetic oligodT-containing beads) was used for RNA-seq library preparation. cDNA preparation and library construction was performed as described by the manufacturer (Illumina, CA, USA) . RNA libraries were sequenced using Genome Analyzer IIx System 50bp single-read method. Llibraries for ChIP-sequencing were generated as described in (Ntziachristos et al., 2012), including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification of the libraries. We performed cluster amplification and 50-nucleotide single-read sequencing using the Illumina HiSeq 2000, following manufacturer’s protocols. Library construction was performed as described by the manufacturer (Illumina, CA, USA)