Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
Dicer KO_ES
strain background
mixed
cell line background
DTCM23 Dicer lox/lox mouse ES cell line
genotype/variation
Dicer KO; Dicer deficient
cell type
embryonic stem cells
chip antibody
None

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
50x10^6 cells were collected by trypsinisation, resuspended in complete media and fixed with 1% formaldehyde. Chromatin isolated from fixed nuclei was sonicated and incubated at 4C overnight with Protein A Dynabeads (Invitrogen) coated with 10ug of anti-Ezh2 antibody (Diagenode). Further steps were carried out according to Seitan et al Nature (2011). Single read high throughput sequencing libraries were prepared from 10ng of ChIP DNA using Illumina ChIP-Seq Sample Prep. Kit and sequenced according to the manufacturer's protocols using Illumina Hiseq 2500

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
133798457
Reads aligned (%)
97.4
Duplicates removed (%)
16.9
Number of peaks
7374 (qval < 1E-05)

mm9

Number of total reads
133798457
Reads aligned (%)
97.2
Duplicates removed (%)
16.8
Number of peaks
6734 (qval < 1E-05)

Base call quality data from DBCLS SRA