Antigen

Antigen Class

TFs and others

Antigen

EZH2

Cell type

Cell type Class

Prostate

Cell type

DU 145

Primary Tissue

Prostate

Tissue Diagnosis

Carcinoma

Sample

source_name

Human prostate cancer cell line

cell line

DU145

tissue

Human prostate cancer cell line

chip antibody

EZH2 (Cell Signaling #5246) / Drosophila H2Av (Active Motif #61686).

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

(ChIP-seq) Chromatin preparation and ChIP experiments were performed with the ChIP-IT High Sensitivity Kit from Active Motif (#53040) according to the manufacturer's instructions. Libraries were prepared according to Illumina instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

36227540

Reads aligned (%)

88.7

Duplicates removed (%)

4.3

Number of peaks

1818 (qval < 1E-05)

hg19

Number of total reads

36227540

Reads aligned (%)

87.9

Duplicates removed (%)

6.5

Number of peaks

1826 (qval < 1E-05)