GSM1463464: VCaP FOXA1 siPIAS1 R1881 rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
FOXA1
Cell type
Cell type Class
Prostate
Cell type
VCaP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
VCaP cells
cell line
Vertebral Cancer of Prostate (VCaP)
cell line origin
bone metastasis of prostate cancer
treated with
10nM R1881 for 2hr
chip antibody
FOXA1
chip antibody vendor
Abcam
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were derived from sonicated nuclei and AR-, PIAS1-, FOXA1-, Pol2 and H3K4me2-DNA complexes were isolated with 1 mg (one microgram) of antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocol, as utilized at the Functional Genomics Unit, Helsinki, Finland, BGI Tech Solutions, Shenzhen, China or at the Gene Core in EMBL, Heidelberg, Germany.