Antigen

Antigen Class

TFs and others

Antigen

Nfatc2

Cell type

Cell type Class

Blood

Cell type

Dendritic Cells

MeSH Description

ANTIGEN-PRESENTING CELLS of dendritic cell morphology found in the LYMPH NODES and other lymphoid tissues.

Sample

source_name

dendritic cells, NFAT1 curdlan V5

cell type

splenic long term dendritic cells

strain

C57BL/6

treatment/control

treatment

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei andNFAT1-v5-DNA complexes were isolated with antibody. Illumina TruSeq DNA version 2 kit according to the manufacturer's protocol, with the following modifications; 30X adaptor dilution, 18 PCR cycles of amplification. Batches of 5-8 samples were sequenced as a multiplex in individual lanes of an Illumina HiSeq 2000 apparatus using a single-end run (1X51) together with the SBS kit v3. Reads were mapped to the mouse genome (version mm9) with bowtie 1 (Langmead et al., 2009) using default parameters.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

32530740

Reads aligned (%)

95.2

Duplicates removed (%)

37.6

Number of peaks

8538 (qval < 1E-05)

mm9

Number of total reads

32530740

Reads aligned (%)

94.9

Duplicates removed (%)

37.6

Number of peaks

8583 (qval < 1E-05)