Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ARNT

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
T47D breast cancer cells
cell line
T47D
antibody
HIF1b
antibody manufacturer
Abcam
antibody catalog number
Ab-2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were constructed by using Illumina ChIP-Seq library preparation kit as instructed

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
203040743
Reads aligned (%)
95.2
Duplicates removed (%)
47.6
Number of peaks
58883 (qval < 1E-05)

hg19

Number of total reads
203040743
Reads aligned (%)
94.5
Duplicates removed (%)
48.9
Number of peaks
57880 (qval < 1E-05)

Base call quality data from DBCLS SRA