Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
HIF1A

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
T47D breast cancer cells
cell line
T47D
antibody
HIF1a
antibody manufacturer
Abcam
antibody catalog number
Ab-2185

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were constructed by using Illumina ChIP-Seq library preparation kit as instructed

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
210067175
Reads aligned (%)
95.0
Duplicates removed (%)
29.8
Number of peaks
108623 (qval < 1E-05)

hg19

Number of total reads
210067175
Reads aligned (%)
94.4
Duplicates removed (%)
30.8
Number of peaks
107663 (qval < 1E-05)

Base call quality data from DBCLS SRA