Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Liver
Cell type
SMMC-7721
NA
NA

Attributes by original data submitter

Sample

source_name
hepatocellular
cell line
SMMC-7721
cell type
hepatocellular carcinoma cell line
genotype/variation
No PTENa/b depletion
chip antibody
H3K4me3 (Millipore, cat#04-745)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and isolated with antibody. Libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

hg38

Number of total reads
18133799
Reads aligned (%)
91.7
Duplicates removed (%)
14.3
Number of peaks
16984 (qval < 1E-05)

hg19

Number of total reads
18133799
Reads aligned (%)
91.1
Duplicates removed (%)
14.6
Number of peaks
16434 (qval < 1E-05)

Base call quality data from DBCLS SRA