Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Irf2bp2

Cell type

Cell type Class
Blood
Cell type
MEL
Tissue
Blood
Lineage
cellLine
Description
Leukemia (K562 analog)

Attributes by original data submitter

Sample

source_name
MEL_IRF2BP2 ChIP
cell type
Mouse erythroleukemia (MEL) cells
chip antibody
Irf2bp2 Absea antibody 10G3
chip antibody info
custom-made

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For endogenous co-immunoprecipitations, MEL nuclear extracts were diluted to reach 100mM KCl salt concentration using Heng 0 buffer (20mM HEPES KOH pH7.9, 20% glycerol, 0.25mM EDTA, 0.05% Np40). For co-IP experiments in MEL cells, 0.5 mg nuclear extract was used per IP. Extracts were treated with 1U Benzonase nuclease (Millipore). For RNA-seq, total RNA was extracted from MEL or E13.5 sorted fetal liver populations using the RNeasy mini kit (Qiagen). Libraries were prepared according to Illumina's standard instructions

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
39990521
Reads aligned (%)
82.9
Duplicates removed (%)
58.4
Number of peaks
5678 (qval < 1E-05)

mm9

Number of total reads
39990521
Reads aligned (%)
82.7
Duplicates removed (%)
58.5
Number of peaks
5742 (qval < 1E-05)

Base call quality data from DBCLS SRA