SRX663359
GSM1447341: Input 3xF LIMB; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Epitope tags

Cell type

Cell type Class
Embryo
Cell type
Embryonic limb
NA
NA

Attributes by original data submitter

Sample

source_name
limb buds
developmental stage
E10.5
antibody
mouse monoclonal anti-FLAG , M2 (Sigma, Cat.-No.: F1804)
strain
NMRI
genotype
Hand23xFLAG

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
A modified ChIP protocol was used based on the protocols described in Kim et al. 2007 (PMID: 18265397) and Visel et al. 2009 (PMID: 19212405) DNA library Prep Kit from New England Biolabs

Sequencing Platform

instrument_model
Illumina HiSeq 2000

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
117852958
Reads aligned (%)
96.6
Duplicates removed (%)
55.4
Number of peaks
33375 (qval < 1E-05)

mm9

Number of total reads
117852958
Reads aligned (%)
95.9
Duplicates removed (%)
55.2
Number of peaks
37082 (qval < 1E-05)

Base call quality data from DBCLS SRA