GSM3986387: GR-356 Ctrl INPUT2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Lung
Cell type
BEAS-2B
Primary Tissue
Lung
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
Beas-2B airway epithelial cells
lentiviral treatment
shCtrl
treatment
dex
ip antibody
none (Input)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) was performed as previously described (PMID: 23508109) with a 5 minute fixation time in 1% methanol-free formaldehyde and 35 cycles (30-second bursts separated by 30-second incubations in ice water) of sonication. Twelve ug of GR-356 (custom-made by Covance) antibody were used for immunoprecipitation. Libraries were prepared using a KAPA HyperPrep kit and sequenced on an Illumina NextSeq using a V2 High Output 1 x 75-cycle single-end kit.