GSM3986385: GR-356 Ctrl 10D2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
Attributes by original data submitter
Beas-2B airway epithelial cells
Sequenced DNA Library
Chromatin immunoprecipitation (ChIP) was performed as previously described (PMID: 23508109) with a 5 minute fixation time in 1% methanol-free formaldehyde and 35 cycles (30-second bursts separated by 30-second incubations in ice water) of sonication. Twelve ug of GR-356 (custom-made by Covance) antibody were used for immunoprecipitation. Libraries were prepared using a KAPA HyperPrep kit and sequenced on an Illumina NextSeq using a V2 High Output 1 x 75-cycle single-end kit.