Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NR3C1

Cell type

Cell type Class
Lung
Cell type
BEAS-2B
Primary Tissue
Lung
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
Beas-2B airway epithelial cells
lentiviral treatment
shCtrl
treatment
vehicle
ip antibody
GR-356

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) was performed as previously described (PMID: 23508109) with a 5 minute fixation time in 1% methanol-free formaldehyde and 35 cycles (30-second bursts separated by 30-second incubations in ice water) of sonication. Twelve ug of GR-356 (custom-made by Covance) antibody were used for immunoprecipitation. Libraries were prepared using a KAPA HyperPrep kit and sequenced on an Illumina NextSeq using a V2 High Output 1 x 75-cycle single-end kit.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
22414631
Reads aligned (%)
15.5
Duplicates removed (%)
41.4
Number of peaks
113 (qval < 1E-05)

hg38

Number of total reads
22414631
Reads aligned (%)
15.9
Duplicates removed (%)
40.3
Number of peaks
115 (qval < 1E-05)

Base call quality data from DBCLS SRA