Antigen

Antigen Class

TFs and others

Antigen

Nr3c1

Cell type

Cell type Class

Blood

Cell type

Macrophages

MeSH Description

The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

Sample

source_name

bone marrow-derived macrophages

strain

back-crossed to the BALB/c

chip antibody

GR, sc-1004, Santa Cruz

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Macrophages were crosslinked in 1 % formaldehyde/PBS, Cell lysis and sonication was performed according to standard ChIP protocol. ChIP-Seq libraries were produced and sequenced according to Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

191356068

Reads aligned (%)

98.0

Duplicates removed (%)

70.9

Number of peaks

3925 (qval < 1E-05)

mm9

Number of total reads

191356068

Reads aligned (%)

97.8

Duplicates removed (%)

71.0

Number of peaks

3733 (qval < 1E-05)