Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Su(H)

Cell type

Cell type Class
Embryo
Cell type
2-4h embryos
NA
NA

Attributes by original data submitter

Sample

source_name
Whole cell embryonic extract
developmental stage
2-4 hour embryo
strain
yw
chip antibody
Santa cruz goat polyclonal #sc-15813
tissue
embryo

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole embryo cell lysates were clarified by centrifugation from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries were prepared according to standard Illumina's protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
39501600
Reads aligned (%)
10.4
Duplicates removed (%)
46.9
Number of peaks
1207 (qval < 1E-05)

dm6

Number of total reads
39501600
Reads aligned (%)
9.7
Duplicates removed (%)
50.2
Number of peaks
2302 (qval < 1E-05)

Base call quality data from DBCLS SRA