Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Blood

Cell type

Acute lymphoid leukemia

NA

NA

Sample

source_name

ALL_H3K4me1

tissue

MLL-AF4 ALL patient blasts

cell type

Bone marrow aspirate from a 15-year old MLL-AF4 ALL patient

chip antibody

Diagenode pAb-194-050

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were washed with PBS and fixed in either 1% FA for 10 minutes (histones) or 2mM DSG for 30 minutes and 1% FA for 30 minutes (EZH2). Cells were sonicated to give fragments of 100-300bp followed by IP. DNA libraries were made using the NEBnext ultra DNA library preparation kit for Illumina (Cat no. E7370).

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

27024826

Reads aligned (%)

97.7

Duplicates removed (%)

1.1

Number of peaks

14408 (qval < 1E-05)

hg19

Number of total reads

27024826

Reads aligned (%)

97.1

Duplicates removed (%)

1.2

Number of peaks

14286 (qval < 1E-05)