Antigen

Antigen Class

TFs and others

Antigen

EZH2

Cell type

Cell type Class

Blood

Cell type

RCH-ACV

Primary Tissue

Blood

Tissue Diagnosis

Leukemia

Sample

source_name

RCH-ACV_EZH2

tissue

Leukemia cell line (RCH-ACV)

cell type

Paediatric pre B-cell line derived from cALL with t(1;19)(q23;p13) translocation

chip antibody

Cell Signalling Technology 5246/4

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were washed with PBS and fixed in either 1% FA for 10 minutes (histones) or 2mM DSG for 30 minutes and 1% FA for 30 minutes (EZH2). Cells were sonicated to give fragments of 100-300bp followed by IP. DNA libraries were made using the NEBnext ultra DNA library preparation kit for Illumina (Cat no. E7370).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

26314500

Reads aligned (%)

84.0

Duplicates removed (%)

9.2

Number of peaks

5581 (qval < 1E-05)

hg19

Number of total reads

26314500

Reads aligned (%)

83.5

Duplicates removed (%)

9.3

Number of peaks

5541 (qval < 1E-05)