Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EZH2

Cell type

Cell type Class
Blood
Cell type
RCH-ACV
Primary Tissue
Blood
Tissue Diagnosis
Leukemia

Attributes by original data submitter

Sample

source_name
RCH-ACV_EZH2
tissue
Leukemia cell line (RCH-ACV)
cell type
Paediatric pre B-cell line derived from cALL with t(1;19)(q23;p13) translocation
chip antibody
Cell Signalling Technology 5246/4

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were washed with PBS and fixed in either 1% FA for 10 minutes (histones) or 2mM DSG for 30 minutes and 1% FA for 30 minutes (EZH2). Cells were sonicated to give fragments of 100-300bp followed by IP. DNA libraries were made using the NEBnext ultra DNA library preparation kit for Illumina (Cat no. E7370).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
26314500
Reads aligned (%)
151.5
Duplicates removed (%)
9.3
Number of peaks
5541 (qval < 1E-05)

hg38

Number of total reads
26314500
Reads aligned (%)
152.6
Duplicates removed (%)
9.2
Number of peaks
5581 (qval < 1E-05)

Base call quality data from DBCLS SRA