Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EZH2

Cell type

Cell type Class
Blood
Cell type
THP-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
THP1_EZH2
tissue
Leukemia cell line (THP1)
cell type
Paediatric monocytic cell line derived from AML M5 with t(9;11)(q21;q23) translocation
chip antibody
Cell Signalling Technology 5246/4

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were washed with PBS and fixed in either 1% FA for 10 minutes (histones) or 2mM DSG for 30 minutes and 1% FA for 30 minutes (EZH2). Cells were sonicated to give fragments of 100-300bp followed by IP. DNA libraries were made using the NEBnext ultra DNA library preparation kit for Illumina (Cat no. E7370).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
27444766
Reads aligned (%)
165.0
Duplicates removed (%)
5.6
Number of peaks
24405 (qval < 1E-05)

hg38

Number of total reads
27444766
Reads aligned (%)
165.7
Duplicates removed (%)
5.5
Number of peaks
24428 (qval < 1E-05)

Base call quality data from DBCLS SRA