Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
THP-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
THP1_H3K27me3_Input
tissue
Leukemia cell line (THP1)
cell type
Paediatric monocytic cell line derived from AML M5 with t(9;11)(q21;q23) translocation
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were washed with PBS and fixed in either 1% FA for 10 minutes (histones) or 2mM DSG for 30 minutes and 1% FA for 30 minutes (EZH2). Cells were sonicated to give fragments of 100-300bp followed by IP. DNA libraries were made using the NEBnext ultra DNA library preparation kit for Illumina (Cat no. E7370).

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
40281638
Reads aligned (%)
98.1
Duplicates removed (%)
1.6
Number of peaks
901 (qval < 1E-05)

hg19

Number of total reads
40281638
Reads aligned (%)
97.2
Duplicates removed (%)
2.0
Number of peaks
658 (qval < 1E-05)

Base call quality data from DBCLS SRA