GSM1443803: CBP hMADS white, day 19; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CREBBP
Cell type
Cell type Class
Adipocyte
Cell type
Adipose-Derived Mesenchymal Stem Cells
NA
NA
Attributes by original data submitter
Sample
source_name
human adipose-derived stem cells
cell line
hMADS-3
cell type
human adipose-derived stem cells
chip antibody
anti-CBP (A-22, sc-369; Santa Cruz)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP experiments were performed in mature hMADS adipocytes essentially as previously described (Siersbæk et al. 2012, MCB, 32: 3452-3463). For cross-linking of chromatin, 0.2 mM disuccinimidyl glutarate (DSG) was applied (Proteochem, Denver, CO) for 45 min followed by cross-linking using 1% formaldehyde for 10 min. ChIP-seq libraries were constructed according to the manufacturer's instructions (Illumina) as described in (Nielsen R, Mandrup S, 2014 Genome-Wide Profiling of Transcription Factor Binding and Epigenetic Marks in Adipocytes by ChIP-seq. Methods in Enzymology 2014, Vol. 537, pp. 261-279).