Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Blood
Cell type
Natural killer cells
NA
NA

Attributes by original data submitter

Sample

source_name
NK Cells
cell type
NK Cells
passages
In vivo FACS purified cells
strain
C57BL/6
chip antibody
H3K4me3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. A new protocol (iChIP) was designed to perform iCHIP from small cell numbers

Sequencing Platform

instrument_model
Illumina HiSeq 1500

mm10

Number of total reads
4093410
Reads aligned (%)
98.5
Duplicates removed (%)
47.1
Number of peaks
11100 (qval < 1E-05)

mm9

Number of total reads
4093410
Reads aligned (%)
98.4
Duplicates removed (%)
47.4
Number of peaks
11113 (qval < 1E-05)

Base call quality data from DBCLS SRA