Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me2

Cell type

Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA

Attributes by original data submitter

Sample

source_name
T CD4+ cells
cell type
T CD4+ cells
passages
In vivo FACS purified cells
strain
C57BL/6
chip antibody
H3K4me2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. A new protocol (iChIP) was designed to perform iCHIP from small cell numbers

Sequencing Platform

instrument_model
Illumina HiSeq 1500

mm10

Number of total reads
15053836
Reads aligned (%)
97.7
Duplicates removed (%)
63.9
Number of peaks
15228 (qval < 1E-05)

mm9

Number of total reads
15053836
Reads aligned (%)
97.5
Duplicates removed (%)
64.0
Number of peaks
15171 (qval < 1E-05)

Base call quality data from DBCLS SRA