Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me1

Cell type

Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA

Attributes by original data submitter

Sample

source_name
T CD4+ cells
cell type
T CD4+ cells
passages
In vivo FACS purified cells
strain
C57BL/6
chip antibody
H3K4me1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. A new protocol (iChIP) was designed to perform iCHIP from small cell numbers

Sequencing Platform

instrument_model
Illumina HiSeq 1500

mm10

Number of total reads
40768572
Reads aligned (%)
98.2
Duplicates removed (%)
48.6
Number of peaks
305 (qval < 1E-05)

mm9

Number of total reads
40768572
Reads aligned (%)
98.0
Duplicates removed (%)
48.7
Number of peaks
312 (qval < 1E-05)

Base call quality data from DBCLS SRA