Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
CUTLL1
NA
NA

Attributes by original data submitter

Sample

source_name
T-ALL Cell line
cell line
CUTLL1
disease state
T-ALL
treatment
gSI (1uM)
chip antibody
H3K27ac (Active motif; Catalog no: 39133)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. libraries were prepared using Kapa Hyper prep kit (Catalog no: KK8500) following manufacturer's guidelines

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
34990601
Reads aligned (%)
98.7
Duplicates removed (%)
8.9
Number of peaks
21029 (qval < 1E-05)

hg19

Number of total reads
34990601
Reads aligned (%)
98.0
Duplicates removed (%)
9.8
Number of peaks
21061 (qval < 1E-05)

Base call quality data from DBCLS SRA