Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CTCF

Cell type

Cell type Class
Blood
Cell type
Jurkat
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic

Attributes by original data submitter

Sample

source_name
T-ALL Cell line
cell line
Jurkat
disease state
T-ALL
treatment
DMSO
chip antibody
CTCF (D31H2; Catalog no: 3418)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. libraries were prepared using Kapa Hyper prep kit (Catalog no: KK8500) following manufacturer's guidelines

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
82300290
Reads aligned (%)
76.8
Duplicates removed (%)
20.4
Number of peaks
37189 (qval < 1E-05)

hg38

Number of total reads
82300290
Reads aligned (%)
78.8
Duplicates removed (%)
19.1
Number of peaks
37597 (qval < 1E-05)

Base call quality data from DBCLS SRA