Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CTCF

Cell type

Cell type Class
Blood
Cell type
T-ALL
NA
NA

Attributes by original data submitter

Sample

source_name
Peripheral blood primary T-ALL cells
tissue
Peripheral blood
disease state
Primary T-ALL
treatment
N/A
chip antibody
CTCF (D31H2; Catalog no: 3418)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. libraries were prepared using Kapa Hyper prep kit (Catalog no: KK8500) following manufacturer's guidelines

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
59224183
Reads aligned (%)
80.9
Duplicates removed (%)
4.6
Number of peaks
34042 (qval < 1E-05)

hg19

Number of total reads
59224183
Reads aligned (%)
80.2
Duplicates removed (%)
5.8
Number of peaks
33812 (qval < 1E-05)

Base call quality data from DBCLS SRA