Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
mouse embryonic stem cells
developmental stage
E14
cell type
mESCs
antibody
none (input)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries were prepared for sequencing using standard Illumina protocols at BGI-Shenzhen (sequencing service provider).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
37335048
Reads aligned (%)
98.0
Duplicates removed (%)
10.3
Number of peaks
534 (qval < 1E-05)

mm9

Number of total reads
37335048
Reads aligned (%)
97.8
Duplicates removed (%)
10.3
Number of peaks
562 (qval < 1E-05)

Base call quality data from DBCLS SRA