Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
mouse embryonic stem cells
cell line
Sim2-expressing cells
clone
B8
antibody
None

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed using the SimpleChIPTM Enzymatic Chromatin IP Kit (Cell Signaling #9003) according to manufacturer’s instructions. Preparation of the libraries for high throughput sequencing was performed using the ChIP-Seq DNA sample Prep Kit (Illumina #IP-102-1001), following the manufacturer’s instructions

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
38423609
Reads aligned (%)
95.8
Duplicates removed (%)
48.7
Number of peaks
289 (qval < 1E-05)

mm9

Number of total reads
38423609
Reads aligned (%)
95.4
Duplicates removed (%)
48.7
Number of peaks
330 (qval < 1E-05)

Base call quality data from DBCLS SRA