GSM3965522: H3K27ac Jurkat-2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Blood
Cell type
Acute T cell leukemia
NA
NA
Attributes by original data submitter
Sample
source_name
T lymphocyte
disease
acute T cell leukemia
germ layer
Mesoderm
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclear lysates were sonicated using a Branson 250 Sonifier (power setting 2, 100% duty cycle for 7 x 30-s intervals). Clarified lysates corresponding to 20 million cells were treated with 1-5ug of antibody against H3K27ac [Abcam #4729]) coupled to Protein G Dynabeads (Life Technologies #10003D, New York). The protein-DNA complexes were washed with RIPA buffer and eluted in 1% SDS TE at 65 °C ChIP DNA sequencing libraries were generated according to Illumina DNA TruSeq DNA Sample Preparation Kit Instructions (Illumina Part # FC-121-2001, San Diego, CA).