Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Large cell lymphoma

NA

NA

Sample

source_name

Lymph node; metastatic to peritoneal effusion

disease

large cell lymphoma; histiocytic

germ layer

Mesoderm

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclear lysates were sonicated using a Branson 250 Sonifier (power setting 2, 100% duty cycle for 7 x 30-s intervals). Clarified lysates corresponding to 20 million cells were treated with 1-5ug of antibody against H3K27ac [Abcam #4729]) coupled to Protein G Dynabeads (Life Technologies #10003D, New York). The protein-DNA complexes were washed with RIPA buffer and eluted in 1% SDS TE at 65 °C ChIP DNA sequencing libraries were generated according to Illumina DNA  TruSeq DNA Sample Preparation Kit Instructions (Illumina Part # FC-121-2001, San Diego,  CA).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

36375308

Reads aligned (%)

98.4

Duplicates removed (%)

4.5

Number of peaks

41078 (qval < 1E-05)

hg19

Number of total reads

36375308

Reads aligned (%)

97.8

Duplicates removed (%)

4.7

Number of peaks

40449 (qval < 1E-05)