Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

Jurkat

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Lymphocytic

Sample

source_name

Jurkat culture cells_H3K27ac

cell line

Jurkat

chip antibody

H3K27ac (Abcam, ab4729)

antibody manufacturer

Abcam

antibody catalog number

ab4729

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin Immunoprecipitation with antibodies against specific histone modifications. Libraries were prepared according to manufacturer's instructions using Illumina TrueSeq ChIP-seq Library Preparation Kit (Ref # 15034288). At least 1ng DNA was used for each library preparation.

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

hg38

Number of total reads

40486693

Reads aligned (%)

96.6

Duplicates removed (%)

9.5

Number of peaks

5848 (qval < 1E-05)

hg19

Number of total reads

40486693

Reads aligned (%)

96.2

Duplicates removed (%)

9.9

Number of peaks

5821 (qval < 1E-05)