Antigen

Antigen Class

TFs and others

Antigen

ARID2

Cell type

Cell type Class

Neural

Cell type

NGP

NA

NA

Sample

source_name

Neuroblastoma

cell line

NGP

cell type

ARID1A-/- #1

chip antiboy

ARID2 (Santa Cruz Biotechnology, #sc166117X)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and target bound fragments were isolated with antibody. Purified immunoprecipitated DNA were prepared for sequencing according to a modified version of the Solexa Genomic DNA protocol. Fragmented DNA was end repaired and subjected to 18 cycles of LM-PCR using oligos provided by Illumina. Amplified fragments between 150 and 300bp (representing shear fragments between 50 and 200nt in length and ~100bp of primer sequence) were isolated by agarose gel electrophoresis and purified.

Sequencing Platform

instrument_model

NextSeq 500

hg19

Number of total reads

34926576

Reads aligned (%)

92.1

Duplicates removed (%)

20.7

Number of peaks

6504 (qval < 1E-05)

hg38

Number of total reads

34926576

Reads aligned (%)

92.7

Duplicates removed (%)

20.1

Number of peaks

6873 (qval < 1E-05)