Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
E(z)

Cell type

Cell type Class
Embryo
Cell type
0-16h embryos
NA
NA

Attributes by original data submitter

Sample

source_name
0-16h embryos
antibody
anti-E(Z), Carrington & Jones, Development (1996).
tissue
0-16h embryos

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Crosslinked lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody, as described in Orlando, et al., Embo J (1998) Sequencing libraries were prepared with the KAPA Library Preparation Kit Illumina series (KK8201) according to the manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
20149351
Reads aligned (%)
84.7
Duplicates removed (%)
11.7
Number of peaks
0 (qval < 1E-05)

dm6

Number of total reads
20149351
Reads aligned (%)
82.1
Duplicates removed (%)
13.9
Number of peaks
2284 (qval < 1E-05)

Base call quality data from DBCLS SRA