Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Breast

Cell type

MDA-MB-468

Primary Tissue

Breast

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MDA-MB-468

cell line

MDA-MB-468

cell type

breast cancer cell line

p53 mutation status

p53 R273H

transfected with

p53 shRNA

chip antibody

Anti-Histone H3 (tri methyl K4)

chip antibody vendor

Abcam

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, cells were crosslinked with formaldehyde (1% final) for 10min at room temperature, and harvested for sonication. Nuclei were extracted and chromatin was sheared to an average size of 200bp using a Diagenode Bioruptor. Sequencing libraries for ChIP-seq were constructd using the NEBNext Ultra kit as per manufacturer's recommended instructions

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

111006347

Reads aligned (%)

99.3

Duplicates removed (%)

15.9

Number of peaks

32455 (qval < 1E-05)

hg19

Number of total reads

111006347

Reads aligned (%)

98.6

Duplicates removed (%)

17.5

Number of peaks

31676 (qval < 1E-05)