Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TP53

Cell type

Cell type Class
Breast
Cell type
HCC70
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
HCC70
cell line
HCC70
cell type
breast cancer cell line
p53 mutation status
p53 R248Q
transfected with
None
chip antibody
Anti-p53, clone DO-1
chip antibody vendor
MABE327

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, cells were crosslinked with formaldehyde (1% final) for 10min at room temperature, and harvested for sonication. Nuclei were extracted and chromatin was sheared to an average size of 200bp using a Diagenode Bioruptor. Sequencing libraries for ChIP-seq were constructd using the NEBNext Ultra kit as per manufacturer's recommended instructions

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
71889441
Reads aligned (%)
92.8
Duplicates removed (%)
5.3
Number of peaks
1357 (qval < 1E-05)

hg38

Number of total reads
71889441
Reads aligned (%)
95.1
Duplicates removed (%)
3.7
Number of peaks
1688 (qval < 1E-05)

Base call quality data from DBCLS SRA