Antigen

Antigen Class

TFs and others

Antigen

TP53

Cell type

Cell type Class

Breast

Cell type

MDA-MB-175-VII

Primary Tissue

Breast

Tissue Diagnosis

Adenocarcinoma Ductal

Sample

source_name

MDA-MB-175VII

cell line

MDA-MB-175VII

cell type

breast cancer cell line

p53 mutation status

p53 WT

transfected with

None

chip antibody

Anti-p53, clone DO-1

chip antibody vendor

MABE327

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, cells were crosslinked with formaldehyde (1% final) for 10min at room temperature, and harvested for sonication. Nuclei were extracted and chromatin was sheared to an average size of 200bp using a Diagenode Bioruptor. Sequencing libraries for ChIP-seq were constructd using the NEBNext Ultra kit as per manufacturer's recommended instructions

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

86293349

Reads aligned (%)

99.2

Duplicates removed (%)

4.2

Number of peaks

2329 (qval < 1E-05)

hg19

Number of total reads

86293349

Reads aligned (%)

98.3

Duplicates removed (%)

5.8

Number of peaks

1865 (qval < 1E-05)