Antigen

Antigen Class

TFs and others

Antigen

Nlrc5

Cell type

Cell type Class

Blood

Cell type

T cells

NA

NA

Sample

source_name

Nlrc5-/- T-cells

strain

C57Bl/6 (H2b) background

cell type

T-cells

genotype/variation

Nlrc5-/-

chip antibody

Rabbit polyclonal anti-mouse NLRC5 (aa 1–139) custom antibody generated by Adipogen

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatine was isolated, fragmented by sonication and immunoprecipitated it with anti Nlrc5 antibody Libraries were prepared according to Illumina's instructions using the Truseq ChIP-seq protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

20710778

Reads aligned (%)

84.4

Duplicates removed (%)

22.5

Number of peaks

311 (qval < 1E-05)

mm9

Number of total reads

20710778

Reads aligned (%)

84.2

Duplicates removed (%)

22.6

Number of peaks

273 (qval < 1E-05)