Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Nlrc5

Cell type

Cell type Class
Blood
Cell type
T cells
NA
NA

Attributes by original data submitter

Sample

source_name
WT T-cells
strain
C57Bl/6 (H2b) background
cell type
T-cells
genotype/variation
WT
chip antibody
Rabbit polyclonal anti-mouse NLRC5 (aa 1–139) custom antibody generated by Adipogen

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatine was isolated, fragmented by sonication and immunoprecipitated it with anti Nlrc5 antibody Libraries were prepared according to Illumina's instructions using the Truseq ChIP-seq protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
30000000
Reads aligned (%)
89.5
Duplicates removed (%)
22.9
Number of peaks
421 (qval < 1E-05)

mm9

Number of total reads
30000000
Reads aligned (%)
89.3
Duplicates removed (%)
23.0
Number of peaks
370 (qval < 1E-05)

Base call quality data from DBCLS SRA