Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NFE2

Cell type

Cell type Class
Blood
Cell type
Erythroblasts
MeSH Description
Immature, nucleated ERYTHROCYTES occupying the stage of ERYTHROPOIESIS that follows formation of ERYTHROID PRECURSOR CELLS and precedes formation of RETICULOCYTES. The normal series is called normoblasts. Cells called MEGALOBLASTS are a pathologic series of erythroblasts.

Attributes by original data submitter

Sample

source_name
Human primary fetal liver proerythroblasts (ProEs), NFE2 ChIP
tissue
fetal liver
cell type
CD34+ HSPC-derived proerythroblasts
chip antibody
NFE2 (Santa Cruz Biotechnology sc-291)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq analysis using the Illumina HiSeq 2000 or HiSeq 2500, 10-20 ng of ChIP DNA was processed for library generation using the ChIP-seq Sample Preparation Kit (Illumina) following the manufacturer's protocol. Raw ChIP-seq data were processed using the Illumina software pipeline. Only ChIP-seq reads that aligned to exactly one location in the reference human genome (UCSC hg18) were retained for downstream data analysis.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
132975398
Reads aligned (%)
19.5
Duplicates removed (%)
77.8
Number of peaks
31338 (qval < 1E-05)

hg38

Number of total reads
132975398
Reads aligned (%)
20.6
Duplicates removed (%)
76.7
Number of peaks
31789 (qval < 1E-05)

Base call quality data from DBCLS SRA