Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC H9

NA

NA

Sample

source_name

H9

cell type

human embryonic stem cells

cell line

H9

condition

CCDC144NL-AS1 KD

passages

35

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For RNA-seq, total RNA of cells was extracted by RNAiso Plus (Takara), according to the manufactures' instructions. For ChIP-seq, lysates were clarified from sonicated nuclei and histone-DNA complexes isolated with antibody RNA libraries were prepared for sequencing using standard Illumina protocols. ChIP-seq libraries were prepared for sequencing using TruSeq Nano DNA Sample Prep Kit (Illumina), according to the manufacter's instructions.

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

hg38

Number of total reads

26309230

Reads aligned (%)

95.5

Duplicates removed (%)

13.4

Number of peaks

1723 (qval < 1E-05)

hg19

Number of total reads

26309230

Reads aligned (%)

94.8

Duplicates removed (%)

13.9

Number of peaks

1332 (qval < 1E-05)