Curated Sample Data


Genome
dm3
Antigen Class
Input control
Antigen
Input control
Cell type Class
Larvae
Cell type
Eye-antennal discs

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
Eye-Antennal disc
genotype
CantonS
sample type
ChIP Input
Stage
Wandering third instar larvae
tissue
Eye-Antennal disc
chip antibody
input

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ATAC-Seq - Nuclei were extracted from 10 eye-antennal discs . FAIRE-Seq - 150 Head complexes were first dissected, fixed and lysed. 200 eye-antennal discs were the uncrosslinked and sonicated, phenol chloroform extraction was used for DNA purification. ChIP-Seq - 750 Head complexes were first fixed, 1000 eye-antennal discs were then dissected and sonicated. Sonicated chromatin was then immunoprecipitated and purified using magnetic beads ATAC-Seq libraries were prepared using a simple PCR reaction with adapters based against the transpon sequence. FAIRE-Seq and ChIP-Seq libraries were prepared using standard Illumina Protocols.

Platform Information


instrument_model
Illumina HiSeq 2000

External Database Query

Logs in read processing pipeline


Number of total reads
23849105
Reads aligned (%)
87.0
Duplicates removed (%)
17.2
Number of peaks
11338 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA